Automation of stimulated gene eductation by electroporation using Pseudomonas putida as an example

In stimulated geneediting, the electroporation of microorganisms causes a change in their genetic material. By selecting modified organisms with advantageous properties, mutations can be produced in a targeted manner. The current process of genome meditation is determined by a large number of manual steps, which provide only a small number of specifically mutated microbes due to the long periods of time required for the mutation of a sufficient number of targets (microbes) as well as the strong process fluctuations. In a new approach, a system for automated genome meditation based on the Multiplex Automated Genome Engineering (MAGE) process will be developed in this project and tested in a prototype study.

In the long run, automation allows processes to be carried out around the clock, allowing more targets to be mutated and greater genomic diversity to be achieved. In addition, process fluctuations can be reduced and stable genome meditations can be realized. Automation offers the possibility to continuously collect data during the process, to improve the process and to adjust process parameters for other types of microbes on the basis of models. Within the framework of this project, a process monitoring system will be implemented which monitors the process to be carried out on the basis of the current growth state of Pseudomonas putida. On the other hand, the necessary biological devices will be integrated into an automated process. A superordinate control and regulation system brings the available information together and ensures that the stimulated gene sedimentation is carried out correctly.